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Kaligrafi Asmaul Husna Pdf Download
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Related Search Tags Download asmaul husna kaligrafi kufi vector · Sanat. 11 Kaligrafi Surat Al Fatihah Download Free PDF Word Doc. Download full- text PDF Al- pdf Asma- ul- Husna.Recognition and removal of exogenous sialic acid in C8 cells by specific membrane sialic acid binding proteins. The recognition and removal of exogenous sialic acid from C8 mouse mastocytoma cells was studied in order to identify the sialic acid binding proteins required for this process. Cultured C8 cells took up added 3H-N-acetylneuraminic acid (3H-Neu5Ac) and 3H-N-acetyl-9-O-acetylneuraminic acid (3H-Neu5,9Ac2) from the medium (Ehrlich, K.A., Pedersen, G.M., and Murray, D.S. (1983) J. Biol. Chem. 258, 4107-4113; K.A. Ehrlich et al., Proc. Natl. Acad. Sci. U.S.A. 80, 849-853 (1983)). Within 2 min of media replacement, C8 cells specifically removed the added sialic acid, while the pH and incubation temperature remained constant. The removal of exogenous sialic acid was blocked by treatment of C8 cells with 4 mM N-acetylneuraminic acid at 4 degrees C. This would indicate that binding of the exogenous sialic acid to C8 cell membrane sialic acid binding proteins is required for sialic acid removal. Asialylated cells when incubated with fresh media were sialylated. The latter results show that these asialylated cells could take up exogenous sialic acid. The result was dependent on the amount of exogenous sialic acid added. Cells desialylated by treatment with neuraminidase followed by reincubation with fresh media did not sialylate after the removal of exogenous sialic acid. This would indicate that specific desialylation of cell membrane sialic acid binding proteins was required for sialylation after removal of the exogenous sialic acid. C8 cells which were desialylated by neuraminidase and preincubated with 4 mM N 3e33713323
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